Research Object Crate for Deepconsensus for Sequel2/2e subreads

Original URL: https://workflowhub.eu/workflows/1075/ro_crate?version=1

# deepconsensus 1.2 snakemake pipeline This snakemake-based workflow takes in a subreads.bam and results in a deepconsensus.fastq - no methylation calls ! The metadata id of the subreads file needs to be: "m[numeric]_[numeric]_[numeric].subreads.bam" Chunking (how many subjobs) and ccs min quality filter can be adjusted in the config.yaml the checkpoint model for deepconsensus1.2 should be accessible like this: gsutil cp -r gs://brain-genomics-public/research/deepconsensus/models/v1.2/model_checkpoint/* "${QS_DIR}"/model/ if that does not work, try to download all at: https://console.cloud.google.com/storage/browser/brain-genomics-public/research/deepconsensus/models?pageState=(%22StorageObjectListTable%22:(%22f%22:%22%255B%255D%22))&prefix=&forceOnObjectsSortingFiltering=false A run example is included in the run_snake.sh Feedback / pull requests welcome! Developed by Daniel Rickert @ WGGC Düsseldorf more to look at: https://www.youtube.com/watch?v=TlWtIao2i9E https://www.nature.com/articles/s41587-022-01435-7

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License
MIT

Contents

Main Workflow: Deepconsensus for Sequel2/2e subreads
Size: 4578 bytes