Research Object Crate for (Hybrid) Metagenomics workflow

**Workflow (hybrid) metagenomic assembly and binning**
- Workflow Illumina Quality: - Sequali (control) - hostile contamination filter - fastp (quality trimming) - Workflow Longread Quality: - NanoPlot (control) - fastplong (quality trimming) - hostile contamination filter - Kraken2 taxonomic classification of FASTQ reads - SPAdes/Flye (Assembly) - Medaka/PyPolCA (Assembly polishing) - QUAST (Assembly quality report) (optional) - Workflow binnning - Metabat2/MaxBin2/SemiBin - Binette - BUSCO - GTDB-Tk (optional) - Workflow Genome-scale metabolic models https://workflowhub.eu/workflows/372 - CarveMe (GEM generation) - MEMOTE (GEM test suite) - SMETANA (Species METabolic interaction ANAlysis) Other UNLOCK workflows on WorkflowHub: https://workflowhub.eu/projects/16/workflows?view=default

**All tool CWL files and other workflows can be found here:**
https://gitlab.com/m-unlock/cwl/
**How to setup and use an UNLOCK workflow:**
https://docs.m-unlock.nl/docs/workflows/setup.html

Author

Bart Nijsse, Jasper Koehorst, Changlin Ke

License

Apache-2.0

Contents