Workflows

What is a Workflow?
2 Workflows visible to you, out of a total of 2
Work-in-progress

Workflow (hybrid) metagenomic assembly and binning

  • Workflow Illumina Quality:
  • Sequali (control)
  • hostile contamination filter
  • fastp (quality trimming)
  • Workflow Longread Quality:
  • NanoPlot (control)
  • fastplong (quality trimming)
  • hostile contamination filter
  • Kraken2 taxonomic classification of FASTQ reads
  • SPAdes/Flye (Assembly)
  • Medaka/PyPolCA (Assembly polishing)
  • QUAST (Assembly quality report)

(optional)

  • Workflow binnning
  • Metabat2/MaxBin2/SemiBin
  • Binette
  • BUSCO

...

Type: Common Workflow Language

Creators: Bart Nijsse, Jasper Koehorst, Changlin Ke

Submitter: Bart Nijsse

DOI: 10.48546/workflowhub.workflow.367.2

Stable

Workflow for Illumina Quality Control and Filtering

Multiple paired datasets will be merged into single paired dataset.

Summary:

  • FastQC on raw data files
  • fastp for read quality trimming
  • BBduk for phiX and (optional) rRNA filtering
  • Kraken2 for taxonomic classification of reads (optional)
  • BBmap for (contamination) filtering using given references (optional)
  • FastQC on filtered (merged) data

Other UNLOCK workflows on WorkflowHub: https://workflowhub.eu/projects/16/workflows?view=default ...

Type: Common Workflow Language

Creators: Bart Nijsse, Jasper Koehorst, Changlin Ke

Submitter: Bart Nijsse

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