NGS Equimolar Pooling Calculator

A web-based tool for calculating equimolar pooling volumes from complex sub-pools for Next-Generation Sequencing (NGS).

About

This calculator helps researchers determine the correct volumes to take from each pool when combining multiple complex sub-pools of NGS libraries for sequencing. It ensures that each individual sample contributes an equal number of molecules to the final sequencing run, resulting in more balanced coverage.

Features

• Calculate ...

Gene_fetch

This tool fetches gene sequences from NCBI databases based on taxonomy IDs (taxids) or taxonomic information. It can retrieve both protein and nucleotide sequences for various genes, including protein-coding genes (e.g., cox1, cytb, rbcl, matk) and rRNA genes (e.g., 16S, 18S).

Feature highlight

• Fetch protein and/or nucleotide sequences from NCBI GenBank database.
• Handles both direct nucleotide sequences and protein-linked nucleotide searches (CDS extraction includes fallback ...

Gene_fetch

This tool fetches gene sequences from NCBI databases based on taxonomy IDs (taxids) or taxonomic information. It can retrieve both protein and nucleotide sequences for various genes, including protein-coding genes (e.g., cox1, cytb, rbcl, matk) and rRNA genes (e.g., 16S, 18S).

Feature highlight

• Fetch protein and/or nucleotide sequences from NCBI GenBank database.
• Handles both direct nucleotide sequences and protein-linked nucleotide searches (CDS extraction includes fallback ...