Genetic Design (Gibson, Golden Gate, LCR)
Version 1

Download RO-Crate Run on Galaxy
Workflow Type: Galaxy

This workflow encodes the top-ranking predicted pathways from the previous workflow into plasmids intended to be expressed in the specified organism. Assembly methods are Gibson, Golden or Ligation Chain Reaction.

SEEK ID: https://workflowhub.eu/workflows/2007?version=1

Inputs

ID Name Description Type
Heterologous pathway #main/Heterologous pathway n/a
  • File
Host taxon ID #main/Host taxon ID n/a
  • int
Taxon IDs of output enzyme sequences #main/Taxon IDs of output enzyme sequences n/a
  • string

Steps

ID Name Description
3 Selenzyme toolshed.g2.bx.psu.edu/repos/tduigou/selenzy/selenzy-wrapper/0.2.0
4 SbmlToSbol toolshed.g2.bx.psu.edu/repos/tduigou/sbml2sbol/sbml2sbol/0.1.13
5 PartsGenie toolshed.g2.bx.psu.edu/repos/tduigou/partsgenie/PartsGenie/1.0.1
6 Design of Experiment toolshed.g2.bx.psu.edu/repos/tduigou/optdoe/optdoe/2.0.2
7 DNA Weaver toolshed.g2.bx.psu.edu/repos/tduigou/dnaweaver/dnaweaver/1.0.2
8 LCR Genie toolshed.g2.bx.psu.edu/repos/tduigou/lcrgenie/LCRGenie/1.0.2

Outputs

ID Name Description Type
DNA Weaver - Assembly Plan #main/DNA Weaver - Assembly Plan n/a
  • File
LCR Genie: Assembly Plan #main/LCR Genie: Assembly Plan n/a
  • File

Version History

Version 1 (earliest) Created 24th Oct 2025 at 14:28 by ramiz khaled

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Frozen Version-1 779a6fa
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Created: 24th Oct 2025 at 14:28

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