Workflows
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Common Workflow Language2
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Bioinformatics2
CWL2
plasmids2
Annotation1
Assembly1
Galaxy1
genbank1
Genomics1
gff31
plasmid1
Python1
Workflows1
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Submitter
Martijn Melissen2
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Independent Teams2
Creator
Martijn Melissen2
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Work-in-progress1
2
Workflows visible to you, out of a total of 2
Work-in-progress
Workflow for long read quality control, contamination filtering, assembly, variant calling and annotation.
Steps:
- Preprocessing of reference file : https://workflowhub.eu/workflows/1818
- LongReadSum before and after filtering (read quality control)
- Filtlong filter on quality and length
- Flye assembly
- Minimap2 mapping of reads and assembly
- Clair3 variant calling of reads
- Freebayes variant calling of assembly
- Optional Bakta annotation of genomes with no reference
- SnpEff building ...
**Workflow for preprocessing a reference file. **
Steps: -When a GenBank file is not provided, it is downloaded from NCBI based on a accession number. -When multiple plasmid GenBank files are provided, they are merged into one file. -When any amount of plasmid GenBank files are provided, the reference is merged with the plasmid GenBank file(s) into one file. A FASTA file is also extracted. -When no plasmid Genbank files are provided, a FASTA file is extracted from the reference GenBank file. -A ...