This document provides a detailed explanation of all the workflows, including their functionalities, problems they address, advantages, disadvantages, implementation requirements, and open points for future versions.
Creator: Daniel Marchan
Submitter: Daniel Marchan
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SPA workflow using cryosparc processing engine
The ultimate-level complexity workflow is one among a collection of workflows designed to address tasks up to CTF estimation. In addition to the functionalities provided by layer 0 and 1 workflows, this workflow aims to enhance the quality of both acquisition images and processing.
Quality control protocols
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Combination of methods
- CTF consensus
- New methods to compare ctf estimations
- CTF xmipp criteria (richer parameters i.e. ice detection)
Advantages:
- Control of ...
The second-level complexity workflow is one among a collection of workflows designed to address tasks up to CTF estimation. In addition to the functionalities provided by the layer 0 workflow, this workflow aims to enhance the quality of acquisition images using quality protocols.
Quality control protocols
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Movie max shift: automatic reject those movies whose frames move more than a given threshold.
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Tilt analysis: quality score based in the Power Spectrum Density (astigmatism and ...
The simplest workflow among a collection of workflows intended to solve tasks up to CTF estimation.
A set of generic and automatic workflows designed to:
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Run on-the-fly and unattended.
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Maintain robust stability for a wide range of samples.
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Covers steps from movies to CTF estimation (for the moment).
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Monitor the acquisition process and provide user feedback.
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Comprise three proposed workflows, each with an additional layer of complexity.
