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      Essential genes detection with Transposon insertion sequencing
Associated Tutorial
This workflows is part of the tutorial Essential genes detection with Transposon insertion sequencing, available in the GTN
Features
- Includes Galaxy Workflow Tests
Thanks to...
Tutorial Author(s): Delphine Lariviere, Bérénice Batut
Inputs
| ID | Name | Description | Type | 
|---|---|---|---|
| Tnseq-Tutorial-reads.fastqsanger.gz | #main/Tnseq-Tutorial-reads.fastqsanger.gz | n/a | 
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| condition_barcodes.fasta | #main/condition_barcodes.fasta | n/a | 
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| construct_barcodes.fasta | #main/construct_barcodes.fasta | n/a | 
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| staph_aur.fasta | #main/staph_aur.fasta | n/a | 
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| staph_aur.gff3 | #main/staph_aur.gff3 | n/a | 
 | 
Steps
| ID | Name | Description | 
|---|---|---|
| 5 | Cutadapt | toolshed.g2.bx.psu.edu/repos/lparsons/cutadapt/cutadapt/1.16.5 | 
| 6 | Nucleotide subsequence search | toolshed.g2.bx.psu.edu/repos/bgruening/find_subsequences/bg_find_subsequences/0.2 | 
| 7 | Convert GFF3 | toolshed.g2.bx.psu.edu/repos/iuc/gff_to_prot/gff_to_prot/3.0.2+galaxy0 | 
| 8 | Cutadapt | toolshed.g2.bx.psu.edu/repos/lparsons/cutadapt/cutadapt/1.16.5 | 
| 9 | Cut | Cut1 | 
| 10 | Cut | Cut1 | 
| 11 | Cutadapt | toolshed.g2.bx.psu.edu/repos/lparsons/cutadapt/cutadapt/1.16.5 | 
| 12 | Compute | toolshed.g2.bx.psu.edu/repos/devteam/column_maker/Add_a_column1/1.3.0 | 
| 13 | Cutadapt | toolshed.g2.bx.psu.edu/repos/lparsons/cutadapt/cutadapt/1.16.5 | 
| 14 | Cut | Cut1 | 
| 15 | Cutadapt | toolshed.g2.bx.psu.edu/repos/lparsons/cutadapt/cutadapt/1.16.5 | 
| 16 | Map with Bowtie for Illumina | toolshed.g2.bx.psu.edu/repos/devteam/bowtie_wrappers/bowtie_wrapper/1.2.0 | 
| 17 | bamCoverage | toolshed.g2.bx.psu.edu/repos/bgruening/deeptools_bam_coverage/deeptools_bam_coverage/3.3.2.0.0 | 
| 18 | Join | toolshed.g2.bx.psu.edu/repos/bgruening/text_processing/tp_easyjoin_tool/1.1.2 | 
| 19 | Join | toolshed.g2.bx.psu.edu/repos/bgruening/text_processing/tp_easyjoin_tool/1.1.2 | 
| 20 | Cut | Cut1 | 
| 21 | Cut | Cut1 | 
| 22 | Sort | toolshed.g2.bx.psu.edu/repos/bgruening/text_processing/tp_sort_header_tool/1.1.1 | 
| 23 | Compute | toolshed.g2.bx.psu.edu/repos/devteam/column_maker/Add_a_column1/1.3.0 | 
| 24 | Cut | Cut1 | 
| 25 | Sort | toolshed.g2.bx.psu.edu/repos/bgruening/text_processing/tp_sort_header_tool/1.1.1 | 
| 26 | Join | toolshed.g2.bx.psu.edu/repos/bgruening/text_processing/tp_easyjoin_tool/1.1.2 | 
| 27 | Compute | toolshed.g2.bx.psu.edu/repos/devteam/column_maker/Add_a_column1/1.3.0 | 
| 28 | Cut | Cut1 | 
| 29 | Sort | toolshed.g2.bx.psu.edu/repos/bgruening/text_processing/tp_sort_header_tool/1.1.1 | 
| 30 | Gumbel | toolshed.g2.bx.psu.edu/repos/iuc/transit_gumbel/transit_gumbel/3.0.2+galaxy0 | 
| 31 | Filter | Filter1 | 
| 32 | Extract Dataset | __EXTRACT_DATASET__ | 
| 33 | Extract Dataset | __EXTRACT_DATASET__ | 
| 34 | Join | toolshed.g2.bx.psu.edu/repos/bgruening/text_processing/tp_easyjoin_tool/1.1.2 | 
| 35 | Join | toolshed.g2.bx.psu.edu/repos/bgruening/text_processing/tp_easyjoin_tool/1.1.2 | 
| 36 | Join | toolshed.g2.bx.psu.edu/repos/bgruening/text_processing/tp_easyjoin_tool/1.1.2 | 
Outputs
| ID | Name | Description | Type | 
|---|---|---|---|
| _anonymous_output_10 | #main/_anonymous_output_10 | n/a | 
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| _anonymous_output_11 | #main/_anonymous_output_11 | n/a | 
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| _anonymous_output_12 | #main/_anonymous_output_12 | n/a | 
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| _anonymous_output_13 | #main/_anonymous_output_13 | n/a | 
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| _anonymous_output_14 | #main/_anonymous_output_14 | n/a | 
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| _anonymous_output_15 | #main/_anonymous_output_15 | n/a | 
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| _anonymous_output_16 | #main/_anonymous_output_16 | n/a | 
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| _anonymous_output_17 | #main/_anonymous_output_17 | n/a | 
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| _anonymous_output_18 | #main/_anonymous_output_18 | n/a | 
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| _anonymous_output_19 | #main/_anonymous_output_19 | n/a | 
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| _anonymous_output_20 | #main/_anonymous_output_20 | n/a | 
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| _anonymous_output_21 | #main/_anonymous_output_21 | n/a | 
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| _anonymous_output_22 | #main/_anonymous_output_22 | n/a | 
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| _anonymous_output_23 | #main/_anonymous_output_23 | n/a | 
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| _anonymous_output_24 | #main/_anonymous_output_24 | n/a | 
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| _anonymous_output_25 | #main/_anonymous_output_25 | n/a | 
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| _anonymous_output_26 | #main/_anonymous_output_26 | n/a | 
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| _anonymous_output_27 | #main/_anonymous_output_27 | n/a | 
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| _anonymous_output_28 | #main/_anonymous_output_28 | n/a | 
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| _anonymous_output_29 | #main/_anonymous_output_29 | n/a | 
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| _anonymous_output_30 | #main/_anonymous_output_30 | n/a | 
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| _anonymous_output_31 | #main/_anonymous_output_31 | n/a | 
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| _anonymous_output_32 | #main/_anonymous_output_32 | n/a | 
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| _anonymous_output_33 | #main/_anonymous_output_33 | n/a | 
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| _anonymous_output_34 | #main/_anonymous_output_34 | n/a | 
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| _anonymous_output_35 | #main/_anonymous_output_35 | n/a | 
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| _anonymous_output_36 | #main/_anonymous_output_36 | n/a | 
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| _anonymous_output_37 | #main/_anonymous_output_37 | n/a | 
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| _anonymous_output_6 | #main/_anonymous_output_6 | n/a | 
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| _anonymous_output_7 | #main/_anonymous_output_7 | n/a | 
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| _anonymous_output_8 | #main/_anonymous_output_8 | n/a | 
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| _anonymous_output_9 | #main/_anonymous_output_9 | n/a | 
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| condition | #main/condition | n/a | 
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| control | #main/control | n/a | 
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| essential_both | #main/essential_both | n/a | 
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| essential_condition | #main/essential_condition | n/a | 
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| essential_control | #main/essential_control | n/a | 
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Version History
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Views: 1188 Downloads: 159 Runs: 0
Created: 2nd Jun 2025 at 10:57
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