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      This workflow generates from only an EBI SCXA reference the metadata for creating an ESet object
Associated Tutorial
This workflows is part of the tutorial Matrix Exchange Format to ESet | Creating a single-cell RNA-seq reference dataset for deconvolution, available in the GTN
Thanks to...
Workflow Author(s): Wendi Bacon, Mehmet Tekman
Tutorial Author(s): Wendi Bacon, Mehmet Tekman
Tutorial Contributor(s): Marisa Loach, Mehmet Tekman, Saskia Hiltemann, Wendi Bacon, Helena Rasche, Pablo Moreno, Julia Jakiela, Pavankumar Videm
Steps
| ID | Name | Description | 
|---|---|---|
| 0 | EBI SCXA Data Retrieval | toolshed.g2.bx.psu.edu/repos/ebi-gxa/retrieve_scxa/retrieve_scxa/v0.0.1+galaxy1 | 
| 1 | Cut | Cut1 | 
| 2 | Add line to file | toolshed.g2.bx.psu.edu/repos/bgruening/add_line_to_file/add_line_to_file/0.1.0 | 
| 3 | Reformatting metadata columns | Adjust this step as necessary toolshed.g2.bx.psu.edu/repos/galaxyp/regex_find_replace/regex1/1.0.2 | 
| 4 | Join two Datasets | Experimental designs often include extra stuff (likely the barcodes not meeting the EBI pre-processing criteria), so this way we streamline down to only those barcodes in the barcodes file. join1 | 
| 5 | Advanced Cut | Gets rid of the duplicated columns toolshed.g2.bx.psu.edu/repos/bgruening/text_processing/tp_cut_tool/1.1.0 | 
Outputs
| ID | Name | Description | Type | 
|---|---|---|---|
| _anonymous_output_1 | #main/_anonymous_output_1 | n/a | 
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| _anonymous_output_2 | #main/_anonymous_output_2 | n/a | 
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| _anonymous_output_3 | #main/_anonymous_output_3 | n/a | 
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| _anonymous_output_4 | #main/_anonymous_output_4 | n/a | 
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| _anonymous_output_5 | #main/_anonymous_output_5 | n/a | 
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| _anonymous_output_6 | #main/_anonymous_output_6 | n/a | 
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| _anonymous_output_7 | #main/_anonymous_output_7 | n/a | 
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| _anonymous_output_8 | #main/_anonymous_output_8 | n/a | 
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| _anonymous_output_9 | #main/_anonymous_output_9 | n/a | 
 | 
Version History
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Views: 1239 Downloads: 181 Runs: 0
Created: 2nd Jun 2025 at 10:58
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