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      Design plasmids encoding predicted pathways by using the BASIC assembly method.
Associated Tutorial
This workflows is part of the tutorial Designing plasmids encoding predicted pathways by using the BASIC assembly method, available in the GTN
Thanks to...
Tutorial Author(s): Kenza Bazi-Kabbaj, Thomas Duigou, Joan Hérisson, Guillaume Gricourt, Ioana Popescu, Jean-Loup Faulon
Inputs
| ID | Name | Description | Type | 
|---|---|---|---|
| DNA-Bot settings | #main/DNA-Bot settings | n/a | 
 | 
| Enzyme taxon IDs | #main/Enzyme taxon IDs | Comma separated values | 
 | 
| Host taxon ID | #main/Host taxon ID | n/a | 
 | 
| Linkers and user parts | #main/Linkers and user parts | n/a | 
 | 
| Pathway (SBML) | #main/Pathway (SBML) | n/a | 
 | 
Steps
| ID | Name | Description | 
|---|---|---|
| 5 | Selenzyme | Performs enzyme selection from a reaction query. toolshed.g2.bx.psu.edu/repos/tduigou/selenzy/selenzy-wrapper/0.2.0 | 
| 6 | BasicDesign | Extracts enzyme IDs from rpSBML files. toolshed.g2.bx.psu.edu/repos/tduigou/rpbasicdesign/rpbasicdesign/0.3.4 | 
| 7 | DNA-Bot | DNA assembly using BASIC on OpenTrons. toolshed.g2.bx.psu.edu/repos/tduigou/dnabot/dnabot/3.1.0 | 
Outputs
| ID | Name | Description | Type | 
|---|---|---|---|
| Uniprot IDs | #main/Uniprot IDs | n/a | 
 | 
| _anonymous_output_1 | #main/_anonymous_output_1 | n/a | 
 | 
| _anonymous_output_2 | #main/_anonymous_output_2 | n/a | 
 | 
| _anonymous_output_3 | #main/_anonymous_output_3 | n/a | 
 | 
| _anonymous_output_4 | #main/_anonymous_output_4 | n/a | 
 | 
| _anonymous_output_5 | #main/_anonymous_output_5 | n/a | 
 | 
| _anonymous_output_6 | #main/_anonymous_output_6 | n/a | 
 | 
Version History
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Views: 1169 Downloads: 165 Runs: 0
Created: 2nd Jun 2025 at 10:59
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