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      Removal of human reads from SARS-CoV-2 sequencing data
Associated Tutorial
This workflows is part of the tutorial Removal of human reads from SARS-CoV-2 sequencing data, available in the GTN
Thanks to...
Tutorial Author(s): Wolfgang Maier
Inputs
| ID | Name | Description | Type | 
|---|---|---|---|
| Input Dataset Collection | #main/Input Dataset Collection | n/a | 
 | 
Steps
| ID | Name | Description | 
|---|---|---|
| 1 | Trimmomatic | toolshed.g2.bx.psu.edu/repos/pjbriggs/trimmomatic/trimmomatic/0.38.1 | 
| 2 | Unzip Collection | __UNZIP_COLLECTION__ | 
| 3 | Map with BWA-MEM | toolshed.g2.bx.psu.edu/repos/devteam/bwa/bwa_mem/0.7.17.2 | 
| 4 | Samtools fastx | toolshed.g2.bx.psu.edu/repos/iuc/samtools_fastx/samtools_fastx/1.9+galaxy1 | 
| 5 | Select | Grep1 | 
| 6 | Replace Text | toolshed.g2.bx.psu.edu/repos/bgruening/text_processing/tp_replace_in_line/1.1.2 | 
| 7 | seqtk_subseq | toolshed.g2.bx.psu.edu/repos/iuc/seqtk/seqtk_subseq/1.3.1 | 
| 8 | seqtk_subseq | toolshed.g2.bx.psu.edu/repos/iuc/seqtk/seqtk_subseq/1.3.1 | 
| 9 | Paired-end collection of cleaned reads | __ZIP_COLLECTION__ | 
Outputs
| ID | Name | Description | Type | 
|---|---|---|---|
| Cleaned Data | #main/Cleaned Data | n/a | 
 | 
| _anonymous_output_10 | #main/_anonymous_output_10 | n/a | 
 | 
| _anonymous_output_11 | #main/_anonymous_output_11 | n/a | 
 | 
| _anonymous_output_2 | #main/_anonymous_output_2 | n/a | 
 | 
| _anonymous_output_3 | #main/_anonymous_output_3 | n/a | 
 | 
| _anonymous_output_4 | #main/_anonymous_output_4 | n/a | 
 | 
| _anonymous_output_5 | #main/_anonymous_output_5 | n/a | 
 | 
| _anonymous_output_6 | #main/_anonymous_output_6 | n/a | 
 | 
| _anonymous_output_7 | #main/_anonymous_output_7 | n/a | 
 | 
| _anonymous_output_8 | #main/_anonymous_output_8 | n/a | 
 | 
| _anonymous_output_9 | #main/_anonymous_output_9 | n/a | 
 | 
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Views: 1081 Downloads: 147 Runs: 0
Created: 2nd Jun 2025 at 11:02
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