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      RAD-Seq Reference-based data analysis
Associated Tutorial
This workflows is part of the tutorial RAD-Seq Reference-based data analysis, available in the GTN
Thanks to...
Tutorial Author(s): Yvan Le Bras
Tutorial Contributor(s): Verena Moosmann, Helena Rasche, Saskia Hiltemann, Nicola Soranzo, Yvan Le Bras, Bérénice Batut, Björn Grüning, William Durand, Niall Beard, Wolfgang Maier
Inputs
| ID | Name | Description | Type | 
|---|---|---|---|
| SRR034310.fastq.gz | EBI SRA: SRR034310 File: ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR034/SRR034310/SRR034310.fastq.gz | n/a | 
 | 
| Barcode_SRR034310.txt | https://zenodo.org/record/1134547/files/Barcode_SRR034310.txt | n/a | 
 | 
| Details_Barcode_Population_SRR034310.txt | https://zenodo.org/record/1134547/files/Details_Barcode_Population_SRR034310.txt | n/a | 
 | 
| Reference_genome_11_chromosomes.fasta | https://zenodo.org/record/1134547/files/Reference_genome_11_chromosomes.fasta | n/a | 
 | 
Steps
| ID | Name | Description | 
|---|---|---|
| 10 | FastQC | toolshed.g2.bx.psu.edu/repos/devteam/fastqc/fastqc/0.71 | 
| 11 | Map with BWA | toolshed.g2.bx.psu.edu/repos/devteam/bwa/bwa/0.7.17.4 | 
| 12 | Select | Grep1 | 
| 13 | Select | Grep1 | 
| 14 | Replace Text | toolshed.g2.bx.psu.edu/repos/bgruening/text_processing/tp_replace_in_line/1.1.1 | 
| 15 | Replace Text | toolshed.g2.bx.psu.edu/repos/bgruening/text_processing/tp_replace_in_line/1.1.1 | 
| 16 | Replace Text | toolshed.g2.bx.psu.edu/repos/bgruening/text_processing/tp_replace_in_line/1.1.1 | 
| 17 | MultiQC | toolshed.g2.bx.psu.edu/repos/iuc/multiqc/multiqc/1.6 | 
| 18 | Replace Text | toolshed.g2.bx.psu.edu/repos/bgruening/text_processing/tp_replace_in_line/1.1.1 | 
| 19 | Replace Text | toolshed.g2.bx.psu.edu/repos/bgruening/text_processing/tp_replace_in_line/1.1.1 | 
| 20 | Add column | addValue | 
| 21 | Concatenate datasets | cat1 | 
| 22 | Cut | Cut1 | 
| 23 | Convert | Convert characters1 | 
| 24 | Regex Replace | toolshed.g2.bx.psu.edu/repos/kellrott/regex_replace/regex_replace/1.0.0 | 
| 25 | Stacks: reference map | toolshed.g2.bx.psu.edu/repos/iuc/stacks_refmap/stacks_refmap/1.46.0 | 
| 26 | Stacks: populations | toolshed.g2.bx.psu.edu/repos/iuc/stacks_populations/stacks_populations/1.46.0 | 
| 27 | Summary Statistics | Summary_Statistics1 | 
| 28 | Sort | toolshed.g2.bx.psu.edu/repos/bgruening/text_processing/tp_sort_header_tool/1.1.1 | 
| 29 | Count | Count1 | 
| 30 | Filter | Filter1 | 
| 4 | Stacks: process radtags | toolshed.g2.bx.psu.edu/repos/iuc/stacks_procrad/stacks_procrad/1.46.0 | 
| 5 | Stacks: process radtags | toolshed.g2.bx.psu.edu/repos/iuc/stacks_procrad/stacks_procrad/1.46.0 | 
| 6 | Stacks: process radtags | toolshed.g2.bx.psu.edu/repos/iuc/stacks_procrad/stacks_procrad/1.46.0 | 
| 7 | Replace Text | toolshed.g2.bx.psu.edu/repos/bgruening/text_processing/tp_replace_in_line/1.1.1 | 
| 8 | Select | Grep1 | 
| 9 | Select | Grep1 | 
Version History
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Views: 1130 Downloads: 152 Runs: 0
Created: 2nd Jun 2025 at 11:03
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