Workflow Type:  Galaxy
        
        
        
  
            
              
                
                     
                
              
            
        
          
            
              
    
      
        
        
    
    
      
        
        
    
            
          
        
        
      
  
    
    
    
  
      
      Metabarcoding/eDNA through Obitools
Associated Tutorial
This workflows is part of the tutorial Metabarcoding/eDNA through Obitools, available in the GTN
Thanks to...
Tutorial Author(s): Coline Royaux, Olivier Norvez, Eric Coissac, Frédéric Boyer, Yvan Le Bras
Tutorial Contributor(s): Helena Rasche, Anne Fouilloux, Yvan Le Bras, Cristóbal Gallardo, Saskia Hiltemann
Funder(s): Pôle National de Données de Biodiversité
Grants(s): Gallantries: Bridging Training Communities in Life Science, Environment and Health
Inputs
| ID | Name | Description | Type | 
|---|---|---|---|
| wolf_tutorial.zip?download=1 | wolf_tutorial.zip?download=1 | n/a | 
 | 
Steps
| ID | Name | Description | 
|---|---|---|
| 1 | Illuminapairedend | toolshed.g2.bx.psu.edu/repos/iuc/obi_illumina_pairend/obi_illumina_pairend/1.2.13 | 
| 2 | Unzip | toolshed.g2.bx.psu.edu/repos/imgteam/unzip/unzip/0.2 | 
| 3 | FastQC | toolshed.g2.bx.psu.edu/repos/devteam/fastqc/fastqc/0.73+galaxy0 | 
| 4 | FASTQ Groomer | toolshed.g2.bx.psu.edu/repos/devteam/fastq_groomer/fastq_groomer/1.1.5 | 
| 5 | obigrep | toolshed.g2.bx.psu.edu/repos/iuc/obi_grep/obi_grep/1.2.13 | 
| 6 | Line/Word/Character count | wc_gnu | 
| 7 | Line/Word/Character count | wc_gnu | 
| 8 | NGSfilter | toolshed.g2.bx.psu.edu/repos/iuc/obi_ngsfilter/obi_ngsfilter/1.2.13 | 
| 9 | obiuniq | toolshed.g2.bx.psu.edu/repos/iuc/obi_uniq/obi_uniq/1.2.13 | 
| 10 | obiannotate | toolshed.g2.bx.psu.edu/repos/iuc/obi_annotate/obi_annotate/1.2.13 | 
| 11 | obistat | toolshed.g2.bx.psu.edu/repos/iuc/obi_stat/obi_stat/1.2.13 | 
| 12 | obigrep | toolshed.g2.bx.psu.edu/repos/iuc/obi_grep/obi_grep/1.2.13 | 
| 13 | obigrep | toolshed.g2.bx.psu.edu/repos/iuc/obi_grep/obi_grep/1.2.13 | 
| 14 | obiclean | toolshed.g2.bx.psu.edu/repos/iuc/obi_clean/obi_clean/1.2.13 | 
| 15 | NCBI BLAST+ blastn | toolshed.g2.bx.psu.edu/repos/devteam/ncbi_blast_plus/ncbi_blastn_wrapper/2.10.1+galaxy0 | 
| 16 | Filter sequences by ID | toolshed.g2.bx.psu.edu/repos/peterjc/seq_filter_by_id/seq_filter_by_id/0.2.7 | 
| 17 | Filter sequences by ID | toolshed.g2.bx.psu.edu/repos/peterjc/seq_filter_by_id/seq_filter_by_id/0.2.7 | 
| 18 | obitab | toolshed.g2.bx.psu.edu/repos/iuc/obi_tab/obi_tab/1.2.13 | 
| 19 | obitab | toolshed.g2.bx.psu.edu/repos/iuc/obi_tab/obi_tab/1.2.13 | 
| 20 | Join two Datasets | join1 | 
| 21 | Join two Datasets | join1 | 
| 22 | Cut | Cut1 | 
| 23 | Filter | Filter1 | 
Version History
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Created: 2nd Jun 2025 at 11:05
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