Workflow Type: Galaxy
Frozen
Work-in-progress
This portion of the workflow produces sets of feature Counts ready for analysis by limma/etc.
Inputs
ID | Name | Description | Type |
---|---|---|---|
UCSC Genome | #main/UCSC Genome | Export of UCSC Genome, just the genes. |
|
mRNA-Seq Reads | #main/mRNA-Seq Reads | Input list of fastqsanger format sequencing data |
|
Steps
ID | Name | Description |
---|---|---|
2 | FastQC | toolshed.g2.bx.psu.edu/repos/devteam/fastqc/fastqc/0.74+galaxy0 |
3 | Cutadapt | toolshed.g2.bx.psu.edu/repos/lparsons/cutadapt/cutadapt/4.4+galaxy0 |
4 | FastQC | toolshed.g2.bx.psu.edu/repos/devteam/fastqc/fastqc/0.74+galaxy0 |
5 | HISAT2 | toolshed.g2.bx.psu.edu/repos/iuc/hisat2/hisat2/2.2.1+galaxy1 |
6 | featureCounts | toolshed.g2.bx.psu.edu/repos/iuc/featurecounts/featurecounts/2.0.3+galaxy1 |
7 | Read Distribution | toolshed.g2.bx.psu.edu/repos/nilesh/rseqc/rseqc_read_distribution/5.0.1+galaxy2 |
8 | Gene Body Coverage (BAM) | toolshed.g2.bx.psu.edu/repos/nilesh/rseqc/rseqc_geneBody_coverage/5.0.1+galaxy2 |
9 | Multi QC raw reads | toolshed.g2.bx.psu.edu/repos/iuc/multiqc/multiqc/1.11+galaxy1 |
Outputs
ID | Name | Description | Type |
---|---|---|---|
html_report | #main/html_report | n/a |
|
output_feature_lengths | #main/output_feature_lengths | n/a |
|
output_short | #main/output_short | n/a |
|
Version History
Version 1 (earliest) Created 19th Dec 2023 at 10:10 by Helena Rasche
Initial commit
Frozen
Version-1
7f98d5d
Creators and Submitter
Creators
Additional credit
Clinical Bioinformatics Unit, Pathology Department, Eramus Medical Center
Submitter
Activity
Views: 2506 Downloads: 403 Runs: 0
Created: 19th Dec 2023 at 10:09
Attributions
None