Workflow Type: Galaxy

This is part of a series of workflows to annotate a genome, tagged with TSI-annotation. These workflows are based on command-line code by Luke Silver, converted into Galaxy Australia workflows.

The workflows can be run in this order:

  • Repeat masking
  • RNAseq QC and read trimming
  • Find transcripts
  • Combine transcripts
  • Extract transcripts
  • Convert formats
  • Fgenesh annotation

For this workflow:

Inputs:

  • assembled-genome.fasta
  • hard-repeat-masked-genome.fasta
  • If using the mRNAs option, the additional inputs required are .cdna, .pro and .dat files.

What it does:

  • This workflow splits the input genomes into single sequences (to decrease computation time), annotates using FgenesH++, and merges the output.

Outputs:

  • genome annotation in gff3 format
  • fasta files of mRNAs, cDNAs and proteins
  • Busco report

Steps

ID Name Description
0 FGENESH split fgenesh_split
1 FGENESH split fgenesh_split
2 FGENESH annotate fgenesh_annotate
3 FGENESH merge fgenesh_merge
4 FGENESH merge fgenesh_merge
5 FGENESH get mRNA or GC fgenesh_get_mrnas_gc
6 FGENESH get mRNA or GC fgenesh_get_mrnas_gc
7 FGENESH get protein fgenesh_get_proteins
8 Busco toolshed.g2.bx.psu.edu/repos/iuc/busco/busco/5.5.0+galaxy0

Version History

Version 3 (latest) Created 17th Nov 2024 at 23:41 by Anna Syme

Changes made 13 Nov 2024: Added correct input files and connected them to the split steps. Added inputs for db selections in the annotation step. Added lineage input to Busco. Added genome annotation stats derived from gff3 output. Connected in assembly.fasta sequences to "get mRNA/cDNA" tools. Expanded this information text and clarified the need for .cdna .pro and .dat files as input.


Frozen Version-3 fe9c08f

Version 2.2 Created 18th Jun 2024 at 10:46 by Anna Syme

Adding .ga file


Frozen Version-2.2 76451e8

Version 2.1 Created 18th Jun 2024 at 10:08 by Anna Syme

add updated workflow image


Frozen Version-2.1 3cebff9

Version 2 Created 18th Jun 2024 at 09:56 by Anna Syme

Updated some labelling for clarity


Frozen Version-2 1b30a7e

Version 1 (earliest) Created 8th May 2024 at 08:28 by Anna Syme

Initial commit


Frozen Version-1 1b30a7e
help Creators and Submitter
Creator
  • Luke Silver
Submitter
Citation
Silver, L. (2024). Fgenesh annotation -TSI. WorkflowHub. https://doi.org/10.48546/WORKFLOWHUB.WORKFLOW.881.2
Activity

Views: 2387   Downloads: 446   Runs: 0

Created: 8th May 2024 at 08:28

Last updated: 17th Nov 2024 at 23:51

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