Workflow Type:  Galaxy
        
        
        
  
        
          
            
              
    
      
        
        
    
    
      
        
        
    
    
      
        
        
    
            
          
        
        
      
  
    
    
    
  
      
  
    
    
    
  
      
  
    
      
        
      
Frozen
    
    
  
      
      Importing single-end multiplexed data (not demultiplexed yet)
Inputs
| ID | Name | Description | Type | 
|---|---|---|---|
| Barcodes | #main/Barcodes | Barcode sequences | 
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| Metadata | #main/Metadata | Metadata | 
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| Metadata parameter | #main/Metadata parameter | Column name of the metadata to be used for demultiplexing | 
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| Reverse complement barcodes | #main/Reverse complement barcodes | Reverse complement barcodes | 
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| Sequences | #main/Sequences | Single-end sequences | 
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Steps
| ID | Name | Description | 
|---|---|---|
| 5 | Input files | qiime2 tools input This step allows the input of the files to be analysed and then generate a qza output, necessary for the QIIME2 pipeline. toolshed.g2.bx.psu.edu/repos/q2d2/qiime2_core__tools__import/qiime2_core__tools__import/2023.5.0+dist.h193f7cc9.3 | 
| 6 | Metadata as artifact | Import the metadata as artifact toolshed.g2.bx.psu.edu/repos/q2d2/qiime2_core__tools__import/qiime2_core__tools__import/2023.5.0+dist.h193f7cc9.3 | 
| 7 | Demultiplex single-end data | qiime2 demux emp-single Demultiplex sequence data generated with the EMP protocol. toolshed.g2.bx.psu.edu/repos/q2d2/qiime2__demux__emp_single/qiime2__demux__emp_single/2023.5.0+q2galaxy.2023.5.0.2 | 
| 8 | Summarize demultiplexed output | qiime2 demux summarize Summarize counts per sample. (Galaxy Version 2023.5.0+q2galaxy.2023.5.0.2) toolshed.g2.bx.psu.edu/repos/q2d2/qiime2__demux__summarize/qiime2__demux__summarize/2023.5.0+q2galaxy.2023.5.0.2 | 
Outputs
| ID | Name | Description | Type | 
|---|---|---|---|
| Demultiplexed single-end data | #main/Demultiplexed single-end data | n/a | 
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| Summary of the demultiplexing | #main/Summary of the demultiplexing | n/a | 
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Version History
v0.2 (earliest) Created 30th May 2024 at 11:35 by WorkflowHub Bot
Updated to v0.2
Frozen
v0.2
      792997b
    
 Creators and SubmitterCreator
Additional credit
Helmholtz-Zentrum für Umweltforschung - UFZ
Submitter
License
Activity
Views: 4555 Downloads: 5093 Runs: 0
Created: 30th May 2024 at 11:35
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