polya_liftover - sc/snRNAseq Snakemake Workflow

A [Snakemake][sm] workflow for using PolyA_DB and UCSC Liftover with Cellranger.

Some genes are not accurately annotated in the reference genome. Here, we use information provide by the [PolyA_DB v3.2][polya] to update the coordinates, then the [USCS Liftover][liftover] tool to update to a more recent genome. Next, we use [Cellranger][cr] to create the reference and count matrix. Finally, by taking advantage of the integrated [Conda][conda] and ...

RNA-Seq pipeline

Here we provide the tools to perform paired end or single read RNA-Seq analysis including raw data quality control, differential expression (DE) analysis and functional annotation. As input files you may use either zipped fastq-files (.fastq.gz) or mapped read data (.bam files). In case of paired end reads, corresponding fastq files should be named using .R1.fastq.gz and .R2.fastq.gz suffixes.

Pipeline Workflow

All analysis steps are illustrated in the pipeline ...

ChIP-Seq pipeline

Here we provide the tools to perform paired end or single read ChIP-Seq analysis including raw data quality control, read mapping, peak calling, differential binding analysis and functional annotation. As input files you may use either zipped fastq-files (.fastq.gz) or mapped read data (.bam files). In case of paired end reads, corresponding fastq files should be named using .R1.fastq.gz and .R2.fastq.gz suffixes.

Pipeline Workflow

All analysis steps are illustrated in ...

DNA-Seq pipeline

Here we provide the tools to perform paired end or single read DNA-Seq analysis including raw data quality control, read mapping, variant calling and variant filtering.

Pipeline Workflow

All analysis steps are illustrated in the pipeline ...

scRNA-Seq pipelines

Here we forge the tools to analyze single cell RNA-Seq experiments. The analysis workflow is based on the Bioconductor packages scater and scran as well as the Bioconductor workflows by Lun ATL, McCarthy DJ, & Marioni JC [*A step-by-step workflow for low-level analysis of single-cell RNA-seq ...

scRNA-Seq pipelines

Here we forge the tools to analyze single cell RNA-Seq experiments. The analysis workflow is based on the Bioconductor packages scater and scran as well as the Bioconductor workflows by Lun ATL, McCarthy DJ, & Marioni JC [*A step-by-step workflow for low-level analysis of single-cell RNA-seq ...

Abstract CWL Automatically generated from the Galaxy workflow file: GTN 'Pangeo 101 for everyone - Introduction to Xarray'.

In this tutorial, we analyze particle matter < 2.5 μm/m3 data from Copernicus Atmosphere Monitoring Service to understand Xarray Galaxy Tools:

• Understand how an Xarray dataset is organized;
• Get metadata from Xarray dataset such as variable names, units, coordinates (latitude, longitude, level), etc;
• Plot an Xarray dataset on a geographical map and learn to customize ...

This workflow extracts 5 different time periods e.g. January- June 2019, 2020 and 2021, July-December 2019 and 2020 over a single selected location. Then statistics (mean, minimum, maximum) are computed. The final products are maximum, minimum and mean.

Description

The workflow takes an input file with Cancer Driver Genes predictions (i.e. the results provided by a participant), computes a set of metrics, and compares them against the data currently stored in OpenEBench within the TCGA community. Two assessment metrics are provided for that predictions. Also, some plots (which are optional) that allow to visualize the performance of the tool are generated. The workflow consists in three standard steps, defined by OpenEBench. The tools needed ...

Summary

This notebook shows how to integrate genomic and image data resources. This notebook looks at the question Which diabetes related genes are expressed in the pancreas?

Steps:

• Query humanmine.org, an integrated database of Homo sapiens genomic data using the intermine API to find the genes.
• Using the list of found genes, search in the Image Data Resource (IDR) for images linked to the genes, tissue and disease.

We use the intermine API and the IDR API

The notebook can be launched ...