Workflow Type:  Galaxy
        
        
        
  
        
          
            
              
    
      
        
        
    
    
      
        
        
    
    
      
        
        
    
            
          
        
        
      
  
    
      
        
      
Frozen
    
    
  
      
      This workflow allows for genome annotation using Maker and evaluates the quality of the annotation.
Inputs
| ID | Name | Description | Type | 
|---|---|---|---|
| Augustus training | Augustus training | Augustus training | 
 | 
| BUSCO lineage | BUSCO lineage | BUSCO lineage | 
 | 
| Genome assembly | Genome assembly | Genome assembly | 
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| Genome sequence | Genome sequence | Genome sequence | 
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| Protein sequences | Protein sequences | Protein sequences | 
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| SNAP training | SNAP training | SNAP training | 
 | 
Steps
| ID | Name | Description | 
|---|---|---|
| 6 | Fasta Statistics | Fasta Statistics on the genome toolshed.g2.bx.psu.edu/repos/iuc/fasta_stats/fasta-stats/2.0 | 
| 7 | BUSCO on the genome sequences | Completeness assessment of the genome using the Busco tool toolshed.g2.bx.psu.edu/repos/iuc/busco/busco/5.7.1+galaxy0 | 
| 8 | Maker | Annotation with Maker toolshed.g2.bx.psu.edu/repos/iuc/maker/maker/2.31.11+galaxy2 | 
| 9 | GFFRead | toolshed.g2.bx.psu.edu/repos/devteam/gffread/gffread/2.2.1.4+galaxy0 | 
| 10 | Genome annotation statistics | Genome annotation statistics on the maker's annotation toolshed.g2.bx.psu.edu/repos/iuc/jcvi_gff_stats/jcvi_gff_stats/0.8.4 | 
| 11 | Map annotation ids | toolshed.g2.bx.psu.edu/repos/iuc/maker_map_ids/maker_map_ids/2.31.11 | 
| 12 | BUSCO on the predicted protein sequences | Completeness assessment of the genome using the Busco tool toolshed.g2.bx.psu.edu/repos/iuc/busco/busco/5.7.1+galaxy0 | 
| 13 | JBrowse | toolshed.g2.bx.psu.edu/repos/iuc/jbrowse/jbrowse/1.16.11+galaxy1 | 
Outputs
| ID | Name | Description | Type | 
|---|---|---|---|
| fasta stats genome | fasta stats genome | n/a | 
 | 
| busco image genome | busco image genome | n/a | 
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| busco sum genome | busco sum genome | n/a | 
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| busco table genome | busco table genome | n/a | 
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| busco gff genome | busco gff genome | n/a | 
 | 
| busco missing genome | busco missing genome | n/a | 
 | 
| maker full | maker full | n/a | 
 | 
| maker gff | maker gff | n/a | 
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| maker evidences | maker evidences | n/a | 
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| gffread exons | gffread exons | n/a | 
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| graphs genome | graphs genome | n/a | 
 | 
| summary genome | summary genome | n/a | 
 | 
| renamed gff3 | renamed gff3 | n/a | 
 | 
| id map | id map | n/a | 
 | 
| busco missing predicted proteins | busco missing predicted proteins | n/a | 
 | 
| busco table predicted proteins | busco table predicted proteins | n/a | 
 | 
| busco gff predicted proteins | busco gff predicted proteins | n/a | 
 | 
| busco sum predicted proteins | busco sum predicted proteins | n/a | 
 | 
| busco image predicted proteins | busco image predicted proteins | n/a | 
 | 
| jbrowse | jbrowse | n/a | 
 | 
Version History
v0.1 (earliest) Created 26th Mar 2025 at 09:50 by WorkflowHub Bot
Updated to v0.1
Frozen
 v0.1
v0.109ce1ab
     Creators and Submitter
 Creators and SubmitterCreator
Submitter
    
    Citation
  
  
  Libouban, R. (2025). annotation-maker/main. WorkflowHub. https://doi.org/10.48546/WORKFLOWHUB.WORKFLOW.1323.1
    License
Activity
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Created: 26th Mar 2025 at 09:50
Last updated: 18th Aug 2025 at 16:39
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 https://orcid.org/0009-0001-4920-9951
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