Purge-duplicates-one-haplotype-VGP6b/main

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Workflow Type: Galaxy

Purge Duplicate Contigs

Purge contigs marked as duplicates by purge_dups in a single haplotype(could be haplotypic duplication or overlap duplication) This workflow is the 6th workflow of the VGP pipeline. It is meant to be run after one of the contigging steps (Workflow 3, 4, or 5)

Inputs

  1. Genomescope model parameters [txt] (Generated by the k-mer profiling workflow)
  2. Hifi long reads - trimmed [fastq] (Generated by Cutadapt in the contigging workflow)
  3. Assembly to purge (e.g. hap1) [fasta] (Generated by the contigging workflow)
  4. K-mer database [meryldb] (Generated by the k-mer profiling workflow)
  5. Estimated Genome Size [txt]
  6. Assembly to leave alone (used for merqury statistics) (e.g. hap2) [fasta] (Generated by the contigging workflow)
  7. Name of un-altered assembly
  8. Name of purged assembly

Outputs

  1. Haplotype 1 purged assembly (Fasta and gfa)
  2. Haplotype 2 purged assembly (Fasta and gfa)
  3. QC: BUSCO report for both assemblies
  4. QC: Merqury report for both assemblies
  5. QC: Assembly statistics for both assemblies
  6. QC: Nx plot for both assemblies
  7. QC: Size plot for both assemblies

SEEK ID: https://workflowhub.eu/workflows/751?version=6

Inputs

ID Name Description Type
Assembly to leave alone (For Merqury comparison) Assembly to leave alone (For Merqury comparison) n/a
  • File
Assembly to purge Assembly to purge n/a
  • File
Estimated genome size - Parameter File Estimated genome size - Parameter File n/a
  • File
Genomescope model parameters Genomescope model parameters n/a
  • File
Meryl Database Meryl Database n/a
  • File
Name of purged assembly Name of purged assembly n/a
  • string?
Name of un-altered assembly Name of un-altered assembly n/a
  • string?
Pacbio Reads Collection - Trimmed Pacbio Reads Collection - Trimmed n/a
  • File[]

Steps

ID Name Description
8 Compute toolshed.g2.bx.psu.edu/repos/devteam/column_maker/Add_a_column1/2.0
9 Map with minimap2 toolshed.g2.bx.psu.edu/repos/iuc/minimap2/minimap2/2.28+galaxy0
10 Purge overlaps toolshed.g2.bx.psu.edu/repos/iuc/purge_dups/purge_dups/1.2.6+galaxy0
11 gfastats toolshed.g2.bx.psu.edu/repos/bgruening/gfastats/gfastats/1.3.6+galaxy0
12 Estimated genome size param_value_from_file
13 Cut Cut1
14 Cut Cut1
15 Map with minimap2 toolshed.g2.bx.psu.edu/repos/iuc/minimap2/minimap2/2.28+galaxy0
16 gfastats_data_prep n/a
17 gfastats toolshed.g2.bx.psu.edu/repos/bgruening/gfastats/gfastats/1.3.6+galaxy0
18 Parse parameter value param_value_from_file
19 Parse parameter value param_value_from_file
20 Text reformatting toolshed.g2.bx.psu.edu/repos/bgruening/text_processing/tp_awk_tool/9.3+galaxy1
21 Purge overlaps toolshed.g2.bx.psu.edu/repos/iuc/purge_dups/purge_dups/1.2.6+galaxy0
22 Purge overlaps toolshed.g2.bx.psu.edu/repos/iuc/purge_dups/purge_dups/1.2.6+galaxy0
23 Remove REPEATs from BED toolshed.g2.bx.psu.edu/repos/bgruening/text_processing/tp_grep_tool/9.3+galaxy1
24 Purge overlaps toolshed.g2.bx.psu.edu/repos/iuc/purge_dups/purge_dups/1.2.6+galaxy0
25 Merqury toolshed.g2.bx.psu.edu/repos/iuc/merqury/merqury/1.3+galaxy4
26 gfastats toolshed.g2.bx.psu.edu/repos/bgruening/gfastats/gfastats/1.3.6+galaxy0
27 Busco toolshed.g2.bx.psu.edu/repos/iuc/busco/busco/5.5.0+galaxy0
28 Convert purged fasta to gfa toolshed.g2.bx.psu.edu/repos/bgruening/gfastats/gfastats/1.3.6+galaxy0
29 gfastats toolshed.g2.bx.psu.edu/repos/bgruening/gfastats/gfastats/1.3.6+galaxy0
30 merqury_QV __EXTRACT_DATASET__
31 output_merqury.spectra-cn.fl __EXTRACT_DATASET__
32 output_merqury.spectra-asm.fl __EXTRACT_DATASET__
33 output_merqury.assembly_01.spectra-cn.fl __EXTRACT_DATASET__
34 merqury_stats __EXTRACT_DATASET__
35 output_merqury.assembly_02.spectra-cn.fl __EXTRACT_DATASET__
36 gfastats_data_prep n/a
37 Text reformatting toolshed.g2.bx.psu.edu/repos/bgruening/text_processing/tp_awk_tool/9.3+galaxy1
38 gfastats_plot n/a
39 Join two Datasets join1
40 Advanced Cut toolshed.g2.bx.psu.edu/repos/bgruening/text_processing/tp_cut_tool/9.3+galaxy1
41 Replace toolshed.g2.bx.psu.edu/repos/bgruening/text_processing/tp_find_and_replace/9.3+galaxy1

Outputs

ID Name Description Type
Cutoffs Cutoffs n/a
  • File
Read Coverage and cutoffs calculation: Histogram plot Read Coverage and cutoffs calculation: Histogram plot n/a
  • File
Removed haplotigs Removed haplotigs n/a
  • File
Purged assembly Purged assembly n/a
  • File
Merqury on Phased assemblies: Images Merqury on Phased assemblies: Images n/a
  • File
Merqury on Phased assemblies: stats Merqury on Phased assemblies: stats n/a
  • File
qv_files qv_files n/a
  • File
Busco on Purged Primary assembly: short summary Busco on Purged Primary assembly: short summary n/a
  • File
Busco on Purged Primary assembly: summary image Busco on Purged Primary assembly: summary image n/a
  • File
Purged assembly (GFA) Purged assembly (GFA) n/a
  • File
Purged assembly statistics Purged assembly statistics n/a
  • File
merqury_QV merqury_QV n/a
  • File
output_merqury.spectra-cn.fl output_merqury.spectra-cn.fl n/a
  • File
output_merqury.spectra-asm.fl output_merqury.spectra-asm.fl n/a
  • File
output_merqury.assembly_01.spectra-cn.fl output_merqury.assembly_01.spectra-cn.fl n/a
  • File
merqury_stats merqury_stats n/a
  • File
output_merqury.assembly_02.spectra-cn.fl output_merqury.assembly_02.spectra-cn.fl n/a
  • File
Nx Plot Nx Plot n/a
  • File
Size Plot Size Plot n/a
  • File
Assembly statistics for both assemblies Assembly statistics for both assemblies n/a
  • File
clean_stats clean_stats n/a
  • File

Version History

v0.7.1 (latest) Created 7th Oct 2024 at 16:34 by WorkflowHub Bot

Updated to v0.7.1


Frozen v0.7.1 cfe3920

v0.7 Created 1st Aug 2024 at 03:02 by WorkflowHub Bot

Updated to v0.7


Frozen v0.7 8cac781

v0.6 Created 30th May 2024 at 11:36 by WorkflowHub Bot

Updated to v0.6


Frozen v0.6 d72d41d

v0.5 Created 23rd Apr 2024 at 03:01 by WorkflowHub Bot

Updated to v0.5


Frozen v0.5 47753b0

v0.4 Created 27th Mar 2024 at 03:02 by WorkflowHub Bot

Updated to v0.4


Frozen v0.4 32c3b9b

v0.3 Created 7th Mar 2024 at 03:02 by WorkflowHub Bot

Updated to v0.3


Frozen v0.3 31f46a9

v0.1 (earliest) Created 15th Feb 2024 at 03:01 by WorkflowHub Bot

Updated to v0.1


Frozen v0.1 49773bd
help Creators and Submitter
Creators
Not specified
Additional credit

Galaxy, VGP

Submitter
Tools
Minimap2
purge_dups
gfastats
Merqury
BUSCO
License
Creative Commons Attribution 4.0 International
Activity

Views: 3047   Downloads: 809   Runs: 0

Created: 15th Feb 2024 at 03:01

Last updated: 17th Aug 2024 at 03:02

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