The workflows in this collection are from the '16S Microbial Analysis with mothur' tutorial for analysis of 16S data (Saskia Hiltemann, Bérénice Batut, Dave Clements), adapted for pipeline use on galaxy australia (Ahmed Mehdi). The workflows developed in galaxy use mothur software package developed by Schloss et al https://pubmed.ncbi.nlm.nih.gov/19801464/.
Please also refer to the 16S tutorials available at Galaxy https://training.galaxyproject.org/training-material/topics/metagenomics/tutorials/mothur-miseq-sop-short/tutorial.html and [https://training.galaxyproject.org/training-material/topics/metagenomics/tutorials/mothur-miseq-sop/tutorial.html
Inputs
| ID | Name | Description | Type | 
|---|---|---|---|
| Cleaned Sequences | #main/Cleaned Sequences | n/a | 
 | 
| Count Table | #main/Count Table | n/a | 
 | 
| Training set FASTA | #main/Training set FASTA | n/a | 
 | 
| Training set Taxonomy | #main/Training set Taxonomy | n/a | 
 | 
Steps
| ID | Name | Description | 
|---|---|---|
| 4 | Classify.seqs | toolshed.g2.bx.psu.edu/repos/iuc/mothur_classify_seqs/mothur_classify_seqs/1.39.5.0 | 
| 5 | Remove.lineage | toolshed.g2.bx.psu.edu/repos/iuc/mothur_remove_lineage/mothur_remove_lineage/1.39.5.0 | 
Outputs
| ID | Name | Description | Type | 
|---|---|---|---|
| _anonymous_output_1 | #main/_anonymous_output_1 | n/a | 
 | 
| _anonymous_output_2 | #main/_anonymous_output_2 | n/a | 
 | 
| _anonymous_output_3 | #main/_anonymous_output_3 | n/a | 
 | 
| _anonymous_output_4 | #main/_anonymous_output_4 | n/a | 
 | 
| _anonymous_output_5 | #main/_anonymous_output_5 | n/a | 
 | 
| _anonymous_output_6 | #main/_anonymous_output_6 | n/a | 
 | 
Version History
Version 1 (earliest) Created 9th Nov 2023 at 05:16 by Sarah Williams
Initial commit
Open
 master
master884afcc
     Creators and Submitter
 Creators and SubmitterCreators
Submitter
Views: 6412 Downloads: 569 Runs: 2
Created: 9th Nov 2023 at 05:16
 Tags
 Tags Attributions
 AttributionsNone
 Collections
 Collections
 Visit source
Visit source Download RO-Crate
Download RO-Crate Run on Galaxy
Run on Galaxy

 16S Microbial Analy...
        16S Microbial Analy...


 https://orcid.org/0000-0003-3803-468X
 https://orcid.org/0000-0003-3803-468X 
