galaxy-SynBioCAD is an open access Galaxy workflow environment to design and build different metabolic routes for the production of a compound of interest. Galaxy-SynBioCAD combines the proven efficiency of RetroPath2.0 and automated script generator to drive robotized workstations for vector assembly and strain transformation.

Annotation: Evaluating and ranking a set of pathways based on multiple metrics. Given a set of pathways generated by RetroPath2.0, this workflow informs the user as to the theoretically best performing ones based on the four criteria (target product flux, thermodynamic feasibility, pathway length, and enzyme availability).

Generating theoretical possible pathways for the production of Lycopene in E.Coli using Retrosynthesis tools

The workflow runs the RetroSynthesis algorithm to generate a collection of heterologous pathways in a host organism of choice, converts them to SBML files, performs analysis on the pathways to then rank the theoretical best performing ones.

Generate possible metabolic routes for the production of a target molecule in an organism of choice

This workflow converts the top-ranking predicted pathways from the "RetroSynthesis" and "Pathway Analysis" workflows to plasmids intended to be expressed in the specified organism

Given a set of pathways generated by RetroPath2.0, this workflow informs the user as to the theoretically best performing ones based on four criteria: FBA, thermodynamic feasibility, length of the pathway, and reaction rule score.