Workflows
What is a Workflow?Filters
The workflow takes a trimmed HiFi reads collection, Forward/Reverse HiC reads, and the max coverage depth (calculated from WF1) to run Hifiasm in HiC phasing mode. It produces both Pri/Alt and Hap1/Hap2 assemblies, and runs all the QC analysis (gfastats, BUSCO, and Merqury). The default Hifiasm purge level is Light (l1).
The ultimate-level complexity workflow is one among a collection of workflows designed to address tasks up to CTF estimation. In addition to the functionalities provided by layer 0 and 1 workflows, this workflow aims to enhance the quality of both acquisition images and processing.
Quality control protocols
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Combination of methods
- CTF consensus
- New methods to compare ctf estimations
- CTF xmipp criteria (richer parameters i.e. ice detection)
Advantages:
- Control of ...
We assume the identifiers of the input list are like: sample_name_replicateID. The identifiers of the output list will be: sample_name
This repository contains the python code to reproduce the experiments in Dłotko, Gurnari "Euler Characteristic Curves and Profiles: a stable shape invariant for big data problems"
This workflow represents the Default ML Pipeline for AutoML feature from MLme. Machine Learning Made Easy (MLme) is a novel tool that simplifies machine learning (ML) for researchers. By integrating four essential functionalities, namely data exploration, AutoML, CustomML, and visualization, MLme fulfills the diverse requirements of researchers while eliminating the need for extensive coding efforts. MLme serves as a valuable resource that empowers researchers of all technical levels to leverage ...
ERGA Protein-coding gene annotation workflow.
Adapted from the work of Sagane Joye:
https://github.com/sdind/genome_annotation_workflow
Prerequisites
The following programs are required to run the workflow and the listed version were tested. It should be noted that older versions of snakemake are not compatible with newer versions of singularity as is noted here: https://github.com/nextflow-io/nextflow/issues/1659.
conda v 23.7.3
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Downloads fastq files for sequencing run accessions provided in a text file using fasterq-dump. Creates one job per listed run accession.
This workflow takes as input SR BAM from ChIP-seq. It calls peaks on each replicate and intersect them. In parallel, each BAM is subsetted to smallest number of reads. Peaks are called using both subsets combined. Only peaks called using a combination of both subsets which have summits intersecting the intersection of both replicates will be kept.
HiFi de novo genome assembly workflow
HiFi-assembly-workflow is a bioinformatics pipeline that can be used to analyse Pacbio CCS reads for de novo genome assembly using PacBio Circular Consensus Sequencing (CCS) reads. This workflow is implemented in Nextflow and has 3 major sections.
Please refer to the following documentation for detailed description of each workflow section:
- [Adapter filtration and pre-assembly quality control ...
Type: Nextflow
Creators: Naga Kasinadhuni, Ziad Al-Bkhetan, Martha Zakrzewski, Kenneth Chan, Uwe Winter, Johan Gustafsson
Submitter: Johan Gustafsson