Workflows
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🧬 Click-qPCR 🧬
An ultra-simple tool for interactive qPCR data analysis developed with R and Shiny.
Overview
Click-qPCR is a user-friendly Shiny web application designed for the straightforward analysis of real-time quantitative PCR (qPCR) data.
This tool is readily accessible via a web browser at https://kubo-azu.shinyapps.io/Click-qPCR/, requiring no local installation for end-users.
It allows users to upload their Cq (quantification cycle) values, perform ΔCq and ΔΔCq calculations, ...
The Spatial Transcriptomics analysis workflow for Xenium data from the PATH2XNAT project tested on the non-diseased lung dataset from 10X genomics. The analysis workflow written in R and executed in the interactive RStudio environment consists of visualizations, clustering, feature selection and cluster annotation.
Training materials elaborating on this analysis workflows can be found in this GitHub repository: https://github.com/HCGB-IGTP/PATH2XNAT/tree/main.
This workflow was developed in the ...
This workflow performs quality and contamination control analysis on assembled contigs to assess bacterial genome quality and taxonomic assignment
Type: Galaxy
Creators: ABRomics , Pierre Marin, Clea Siguret, abromics-consortium
Submitter: WorkflowHub Bot
Short paired-end read analysis to provide quality analysis, read cleaning and taxonomy assignation directly from raw reads
Type: Galaxy
Creators: ABRomics , Pierre Marin, Clea Siguret, abromics-consortium
Submitter: WorkflowHub Bot
Projet de Hackathon du master AMI2B 2022 - Pipeline d'analyse RNA-Seq écrit en Nextflow
Type: Nextflow
Creators: George , George-Marchment , ClemenceS , ambrebaumann , Lindsay Goulet, Lindsay-Goulet , Ubuntu , Ambre , Lindsay GOULET
Submitter: Jaffar GURA
Projet de Hackathon du master AMI2B 2022 - Pipeline d'analyse RNA-Seq écrit en Nextflow
Type: Nextflow
Creators: George , George-Marchment , ClemenceS , ambrebaumann , Lindsay Goulet, Lindsay-Goulet , Ubuntu , Ambre , Lindsay GOULET
Submitter: Jaffar GURA
This workflow processes the CMO fastqs with CITE-seq-Count and include the translation step required for cellPlex processing. In parallel it processes the Gene Expresion fastqs with STARsolo, filter cells with DropletUtils and reformat all outputs to be easily used by the function 'Read10X' from Seurat.
Type: Galaxy
Creators: Lucille Delisle, Mehmet Tekman, Hans-Rudolf Hotz, Daniel Blankenberg, Wendi Bacon
Submitter: WorkflowHub Bot
Complete ChIP-seq analysis for single-end sequencing data. Processes raw FASTQ files through adapter removal (cutadapt), alignment to reference genome (Bowtie2), and quality filtering (MAPQ >= 30). Peak calling with MACS2 uses either a fixed extension parameter or built-in model to identify protein-DNA binding sites. Generates alignment files, peak calls, and quality metrics for downstream analysis.
Complete ChIP-seq analysis for paired-end sequencing data. Processes raw FASTQ files through adapter removal (cutadapt), alignment to reference genome (Bowtie2), and stringent quality filtering (MAPQ >= 30, concordant pairs only). Peak calling with MACS2 optimized for paired-end reads identifies protein-DNA binding sites. Generates alignment files, peak calls, and quality metrics for downstream analysis.
**Workflow (hybrid) metagenomic assembly and binning **
- Workflow Illumina Quality: https://workflowhub.eu/workflows/336?version=1
- FastQC (control)
- fastp (quality trimming)
- kraken2 (taxonomy)
- bbmap contamination filter
- Workflow Longread Quality:
- NanoPlot (control)
- filtlong (quality trimming)
- kraken2 (taxonomy)
- minimap2 contamination filter
- Kraken2 taxonomic classification of FASTQ reads
- SPAdes/Flye (Assembly)
- Pilon/Medaka/PyPolCA (Assembly polishing)
- QUAST (Assembly ...
Type: Common Workflow Language
Creators: Bart Nijsse, Jasper Koehorst, Changlin Ke
Submitter: Bart Nijsse