Workflows
What is a Workflow?Filters
Variant calling and consensus sequence generation for batches of Illumina PE sequenced viruses with uncomplicated and stable genome structure (like e.g. Morbilliviruses).
Cloning simulation workflow for sequences present in DB
Run the GoldenGate cloning simulation for a list of constructs in a CSV file and interact with a database
steps:
input: csv file (without header) : The CSV file should contain the constraints line by line in the first column, along with their associated fragments on each line. This data will be passed to the seq_from_DB tool.
workflow_1 Parameter_Maystro
- Distribute workflow parameters on the workflow tools
- Parameters ...
COVID-19: variation analysis on ARTIC ONT data
This workflow for ONT-sequenced ARTIC data is modeled after the alignment/variant-calling steps of the ARTIC pipeline. It performs, essentially, the same steps as that pipeline’s minion command, i.e. read mapping with minimap2 and variant calling with medaka. Like the Illumina ARTIC workflow it uses ivar for primer trimming. Since ONT-sequenced reads have a much ...
This workflow takes as input a SRA_manifest from SRA Run Selector and will generate one fastq file or fastq pair of file for each experiment (concatenated multiple runs if necessary). Output will be relabelled to match the column specified by the user.
This workflow performs subtyping and consensus sequence generation for batches of Illumina PE sequenced Influenza A isolates.
Type: Galaxy
Creators: Viktoria Isabel Schwarz, Wolfgang Maier, Aaron Kolbecher, Saim Momin
Submitter: WorkflowHub Bot
A workflow for the analysis of pox virus genomes sequenced as half-genomes (for ITR resolution) in a tiled-amplicon approach
This workflows performs single end read mapping with bowtie2 followed by sensitive variant calling across a wide range of AFs with lofreq
Build a consensus sequence from FILTER PASS variants with intrasample allele-frequency above a configurable consensus threshold. Hard-mask regions with low coverage (but not consensus variants within them) and ambiguous sites.
This workflow takes a VCF dataset of variants produced by any of the *-variant-calling workflows in https://github.com/galaxyproject/iwc/tree/main/workflows/sars-cov-2-variant-calling and generates tabular lists of variants by Samples and by Variant, and an overview plot of variants and their allele-frequencies.
Overview
Developmental version of MSC: This github page contains developmental version of R package for Multi-scale clustering (MSC) to perform single-cell transcriptome clustering. The manuscript is currently under review.
Installation:
MEGENA needs to be installed, prior to MSC installation: library(devtools); install_github("songw01/MEGENA");
For installation for developmental github version: library(devtools); install_github("songlabcodes/MSC");
Vignettes [PBMC 8k ...