Workflows
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Evaluation of Pacbio Hifi Reads and genome profiling. Create Meryl Database used for the estimation of assembly parameters and quality control with Merqury. Part of the VGP pipeline.
name: "CI" on: [push, pull_request]
jobs: build: strategy: fail-fast: false matrix: ruby: ["2.5", "2.6", "2.7", "3.0"]
runs-on: macos-latest steps:
Setup env
- uses: actions/checkout@v2
- uses: ruby/setup-ruby@v1 with: ruby-version: "${{ matrix.ruby }}"
Show env
- name: Show macOS version run: sw_vers
- name: Show env versions run: | ruby --version bundler --version echo $HOME
Prepare
- name: Install bundler 2.2.20 run: gem install bundler -v "~> 2.2.20"
- name: Install ruby dependencies ...
Associated Tutorial
This workflows is part of the tutorial Assembly of the mitochondrial genome from PacBio HiFi reads, available in the GTN
Features
- Includes Galaxy Workflow Tests
Thanks to...
Workflow Author(s): VGP, Galaxy
**Tutorial ...
Associated Tutorial
This workflows is part of the tutorial Proteogenomics 2: Database Search, available in the GTN
Features
- Includes Galaxy Workflow Tests
- Includes a Galaxy Workflow Report ...
Soil-WAter Quality model (SWAQ)
There are three main types of files:
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*_AppPatt.R Used for building country specific Gemup (GEnaralised Mapped Usage Patterns). Only for CZ there is also CZ_PPPusage.R used for cleaning PPP usage data.
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*_swaq_farm.R Used for calculating PEC and RQ at field level. Only CZ script is completed for the moment but NL and DK should follow similar template.
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*_swaq_bas.R Used for calculating PEC and RQ at river catchment level. All scripts are completed. Only ...
Workflow (hybrid) metagenomic assembly and binning
- Workflow Illumina Quality: https://workflowhub.eu/workflows/336
- Sequali (control)
- hostile contamination filter
- fastp (quality trimming)
- Workflow Longread Quality: https://workflowhub.eu/workflows/337
- NanoPlot (control)
- fastplong (quality trimming)
- hostile contamination filter
- Kraken2 taxonomic classification of FASTQ reads
- SPAdes/Flye (Assembly) ...
Type: Common Workflow Language
Creators: Bart Nijsse, Jasper Koehorst, Changlin Ke
Submitter: Bart Nijsse
Assembly polishing; can run alone or as part of a combined workflow for large genome assembly.
- What it does: Polishes (corrects) an assembly, using long reads (with the tools Racon and Medaka) and short reads (with the tool Racon). (Note: medaka is only for nanopore reads, not PacBio reads).
- Inputs: assembly to be polished: assembly.fasta; long reads - the same set used in the assembly (e.g. may be raw or filtered) fastq.gz format; short reads, R1 only, in fastq.gz format
- Outputs: ...
Post-genome assembly quality control workflow using Quast, BUSCO, Meryl, Merqury and Fasta Statistics, with updates November 2024.
Workflow inputs: reads as fastqsanger.gz (not fastq.gz), and primary assembly.fasta. (To change reads format: click on the pencil icon next to the file in the Galaxy history, then "Datatypes", then set "New type" as fastqsanger.gz). Note: the reads should be those that were used for the assembly (i.e., the filtered/cleaned reads), not the raw reads.
What it does: ...
Type: Galaxy
Creators: Kate Farquharson, Gareth Price, Simon Tang, Anna Syme, Gareth Price, Anna Syme
Submitters: Johan Gustafsson, Anna Syme
ORBiT
A Nextflow workflow for analysing Oxford Nanopore Technologies (ONT) RNAseq direct read sequening (DRS) or cDNA data.
This workflow emphasises sensitivity to detect rare and novel features within the data. Multiple aspects of this workflow are tailored to enhance sensitivity:
- Alignment to reference genome rather than transcriptome
- Multiple tools per analysis type (n = 2 isoforms, n = 3 fusions)
- Reads quantification tools capable of detecting novel isoforms, and counting at the isoform ...
Type: Nextflow
Creators: Cali Willet, Amarinder Thind, Michael Geaghan, Mitchell O'Brien, Madison Gonebale, Marina Kennerson
Submitter: Georgina Samaha
This Workflow transcribes a video or audio with multiple speakers. After transcription, it allocates the names of the speakers and groups and cleans passages from the two main speakers for further analysis.
Associated Tutorial
This workflows is part of the tutorial Transcribing Audio and Video files with Automated Speech Recognition, available in the ...