Workflows

What is a Workflow?
1321 Workflows visible to you, out of a total of 1412
Stable

Soil Metagenome Pipeline

Soil Metagenome Pipeline is a modular, Nextflow DSL2 workflow for assembling, polishing, binning, annotating, and functionally characterizing complex soil metagenomes. It orchestrates state-of-the-art tools for long- and short-read metagenomics, generates high-quality MAGs, assigns taxonomy, and screens for biosynthetic gene clusters (BGCs).

What it does

  • Assembles long-read metagenomes (e.g., ONT) with Flye and optionally polishes with Medaka and/or NextPolish using ...

Type: Nextflow

Creator: Caner Bagci

Submitter: Caner Bağcı

DOI: 10.48546/workflowhub.workflow.1960.1

Stable

The workflow main goal is to align DNA-Seq read files with BWA-MEM , with optional adapter trimming, UMI consensus calling, duplicate marking. Finally it runs Multi QC to gather tool metrics. The workflow was designed to be run in the SeqUIa (http://cfb.ceitec.muni.cz/sequia) application.

Type: Snakemake

Creators: None

Submitter: Nicolas Blavet

Stable

The workflow main goal is to run divers tools to obtain alignment quality metrics from RNA-Seq, DNA-Seq, and ChIP-Seq using Picard, Qualimap, Samtools, dupradar, biotype analysis, FastQ Screen, RSeQC or Phantom peak. Finally it runs Multi QC to gather tool metrics. The workflow was designed to be run in the SeqUIa (http://cfb.ceitec.muni.cz/sequia) application.

Type: Snakemake

Creators: None

Submitter: Nicolas Blavet

Stable

The workflow main goal is to count reads aligned or pseudo-aligned to reference genome annotation using featureCount, HTSeq-count, RSEM, Kallisto or Salmon. Finally it runs Multi QC to gather tool metrics. The workflow was designed to be run in the SeqUIa (http://cfb.ceitec.muni.cz/sequia) application.

Type: Snakemake

Creators: None

Submitter: Nicolas Blavet

Stable

The workflow main goal is to align RNA-Seq read files with STAR and to check for duplicates using either Picard Markduplicates or UMI-tools. Finally it runs Multi QC to gather tool metrics. The workflow was designed to be run in the SeqUIa (http://cfb.ceitec.muni.cz/sequia) application.

Type: Snakemake

Creators: None

Submitter: Nicolas Blavet

Stable

GitHub Actions CI Status GitHub Actions Linting StatusAWS CI[![Cite ...

Type: Nextflow

Creators: Evangelos Karatzas, Martin Beracochea

Submitter: Evangelos Karatzas

DOI: 10.48546/workflowhub.workflow.1954.1

The workflow serves as a short introduction to Galaxy for users from the Humanities who mostly work with texts. The workflow compares two texts, visualises the differences with 'diff' and a wordcloud and extracts selected passaged for further analysis.

Associated Tutorial

This workflows is part of the tutorial Introduction to Digital Humanities in Galaxy, available in ...

Type: Galaxy

Creators: None

Submitter: GTN Bot

Workflow for quality assessment of paired reads and classification using NGTax 2.0 and functional annotation using picrust2.
In addition files are exported to their respective subfolders for easier data management in a later stage.

Steps:

  • Quality plots (FastQC)
  • NG-TAX 2 High-throughput Amplicon Analysis
  • PICRUSt 2 - Function prediction from marker gene sequences
  • Export module for ngtax

Type: Common Workflow Language

Creators: Bart Nijsse, Jasper Koehorst

Submitter: Jasper Koehorst

DOI: 10.48546/workflowhub.workflow.154.2

Work-in-progress

Workflow for quality assessment and taxonomic classification of amplicon long read sequences. In addition files are exported to their respective subfolders for easier data management in a later stage.

Inputs are expected to be basecalled fastq files

Steps:

  • NanoPlot read quality control, before and after filtering
  • fastplong read quality and length filtering
  • Emu abundance; species-level taxonomic abundance for full-length 16S read

Type: Common Workflow Language

Creators: Bart Nijsse, Jasper Koehorst

Submitter: Bart Nijsse

Work-in-progress

Workflow (hybrid) metagenomic assembly and binning

  • Workflow Illumina Quality:
  • Sequali (control)
  • hostile contamination filter
  • fastp (quality trimming)
  • Workflow Longread Quality:
  • NanoPlot (control)
  • fastplong (quality trimming)
  • hostile contamination filter
  • Kraken2 taxonomic classification of FASTQ reads
  • SPAdes/Flye (Assembly)
  • Medaka/PyPolCA (Assembly polishing)
  • QUAST (Assembly quality report)

(optional)

  • Workflow binnning
  • Metabat2/MaxBin2/SemiBin
  • Binette
  • BUSCO

...

Type: Common Workflow Language

Creators: Bart Nijsse, Jasper Koehorst, Changlin Ke

Submitter: Bart Nijsse

DOI: 10.48546/workflowhub.workflow.367.2

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