Workflows
What is a Workflow?Filters
AnnoAudit - Annotation Auditor
AnnoAudit is a robust Nextflow pipeline designed to evaluate the quality of genomic annotations through a multifaceted approach.
Overview of the workflow
The workflow assess the annotation quality based on different criteria:
- Protein evidence support
- RNASeq evidence support
- Statistics of the predictions (i.e., gene length, exon number, etc.)
- Ortholog analysis (BUSCO, OMArk)
Input data
- Reference genome
genome.[.fna, .fa, .fasta]
- Annotation ...
Prostate cancer classification workflow
This workflow segments tissue regions and classifies prostate cancer on H&E whole slide images, using AI. It consists of three steps:
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low-resolution tissue segmentation to select areas for further processing;
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high-resolution tissue segmentation to refine borders - it uses step 1 as input;
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high-resolution normal/cancer classification - it uses step 1 as input.
Tissue segmentation workflow
This workflow performs tissue segmentation on H&E whole slide images using AI.
This workflow generates Hi-C contact maps for genome assemblies in the Pretext format. It is compatible with one or 2 haplotypes. It includes tracks for PacBio read coverage, Gaps, and telomeres. The Pretext files can be open in PretextView for the manual curation of genome assemblies.
Generate Nx and Size plot for multiple assemblies
Inputs
Collection of fasta files. The name of each item in the collection will be used as label for the Nx and Size plots.
Outputs
- Nx plot
- Size plot
Microbiome - Variant calling and Consensus Building
Build a consensus sequence from FILTER PASS variants with intrasample allele-frequency above a configurable consensus threshold. Hard-mask regions with low coverage (but not consensus variants within them) and ambiguous sites.