Workflow Type: Common Workflow Language
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Workflow for gene set enrichment analsysis (GSEA) and co-expression analysis (WGCNA) on transcriptomics data to analyze pathways affected in Porto-Sinusoidal Vascular Disease.
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Inputs
ID | Name | Description | Type |
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raw_counts | Microarray data | Illumina beadchip raw gene expression data -GSE77627 |
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bgxfile | Illumina beadchip array file | Illumina HumanHT-12 WG-DASL V4.0 R2 expression beadchip |
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clinical_data | Clinical data | Clinical phenotype data |
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Steps
ID | Name | Description |
---|---|---|
pre_processing | Data pre-processing | Pre-processing input data |
quality_control | Quality control | Quality control of input data |
DEG | Differential gene expression analysis | Differential gene expression analysis using limma |
wgcna | Weighted gene co-expression network analysis | Weighted gene co-expression network analysis for module identification |
gsea_analysis | Gene set enrichment analysis and visualisation | Gene set enrichment analysis and visualisation in Cytoscape |
ME_network | Module eigenegene correlation network | Module eigenegene correlation network and visualisation in Cytoscape |
module_ora | Module enrichment analysis | Module enrichment analysis with significantly correlating modules |
Outputs
ID | Name | Description | Type |
---|---|---|---|
gsea_node_table | GSEA node table | Node table for GSEA network result visualisation in Cytoscape |
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gsea_edge_table | GSEA edge table | Edge table for GSEA network result visualisation in Cytoscape |
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net_data_table | Network data table | GSEA network data table |
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add_net_data_table | Additional network data table | GSEA network additional visualisation table |
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module_eigengene_net_nodetable | Module eigengene node data | Module eigenegene correlation network node table in Cytoscape |
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module_eigengene_net_edgetable | Module eigengene edge data | Module eigenegene correlation network edge table in Cytoscape |
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Version History
Version 1 (earliest) Created 11th Jun 2024 at 15:13 by Aishwarya Iyer
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Citation
Iyer, A., & Ehrhart, F. (2024). Porto-Sinusoidal Vascular Disease transcriptomics analysis workflow. WorkflowHub. https://doi.org/10.48546/WORKFLOWHUB.WORKFLOW.1040.1
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Views: 1237 Downloads: 184
Created: 11th Jun 2024 at 15:13
Last updated: 14th Jun 2024 at 13:23
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