Differential peak analysis with SnapATAC2
Version 1

Workflow Type: Galaxy

This workflow takes a cell-type-annotated AnnData object (processed with SnapATAC2) and performs peak calling with MACS3 on the cell types. Next, a cell-by-peak matrix is constructed and differential accessibility tests are performed for comparison of either two cell types or one cell type with a background of all other cells. Lastly, differentially accessible marker regions for each cell type are identified.

Inputs

ID Name Description Type
1 Cell-type #1 n/a
  • string
2 Cell-type #2 n/a
  • string
Cell-type Annotated AnnData Cell-type Annotated AnnData n/a
  • File
chrom_sizes_new.txt chrom_sizes_new.txt n/a
  • File

Steps

ID Name Description
4 macs3 toolshed.g2.bx.psu.edu/repos/iuc/snapatac2_peaks_and_motif/snapatac2_peaks_and_motif/2.6.4+galaxy1
5 cell-types_UMAP-plot toolshed.g2.bx.psu.edu/repos/iuc/snapatac2_plotting/snapatac2_plotting/2.6.4+galaxy1
6 merge_peaks toolshed.g2.bx.psu.edu/repos/iuc/snapatac2_peaks_and_motif/snapatac2_peaks_and_motif/2.6.4+galaxy1
7 make_peak_matrix toolshed.g2.bx.psu.edu/repos/iuc/snapatac2_peaks_and_motif/snapatac2_peaks_and_motif/2.6.4+galaxy1
8 Differential Expression between two cell types Compares differentially accessible peaks between two cell types (f.ex. Memory B and Naive B cells) toolshed.g2.bx.psu.edu/repos/iuc/snapatac2_peaks_and_motif/snapatac2_peaks_and_motif/2.6.4+galaxy1
9 Differential Expression compared to all cell types Compares differentially accessible peaks between a cell type (f.ex. Naive B cell) and 30 random "background" cells of the other cell types toolshed.g2.bx.psu.edu/repos/iuc/snapatac2_peaks_and_motif/snapatac2_peaks_and_motif/2.6.4+galaxy1
10 marker_regions toolshed.g2.bx.psu.edu/repos/iuc/snapatac2_peaks_and_motif/snapatac2_peaks_and_motif/2.6.4+galaxy1

Outputs

ID Name Description Type
marker_regions marker_regions n/a
  • File
marker_regions-plot marker_regions-plot n/a
  • File

Version History

Version 1 (earliest) Created 2nd Aug 2024 at 19:23 by Timon Schlegel

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Created: 2nd Aug 2024 at 19:23

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