The Spatial Transcriptomics analysis workflow for Xenium data from the PATH2XNAT project tested on the non-diseased lung dataset from 10X genomics. The analysis workflow written in R and executed in the interactive RStudio environment consists of visualizations, clustering, feature selection and cluster annotation.
Training materials elaborating on this analysis workflows can be found in this GitHub repository: https://github.com/HCGB-IGTP/PATH2XNAT/tree/main.
This workflow was developed in the PATH2XNAT joint research action within the ISIDORe project. ISIDORe has received funding from the European Union’s Horizon Europe research and innovation programme under grant agreement number 101046133.
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Inputs
| ID | Name | Description | Type | 
|---|---|---|---|
| Non_diseased_lung_minimal_RDS | n/a | n/a | 
 | 
| Non_diseased_lung_minimal_zip | n/a | n/a | 
 | 
| Xenium_hLung_v1_metadata_csv | n/a | n/a | 
 | 
| test_RData | n/a | n/a | 
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| test_RHistory | n/a | n/a | 
 | 
| workspace_RData | n/a | n/a | 
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| workspace_RHistory | n/a | n/a | 
 | 
Steps
| ID | Name | Description | 
|---|---|---|
| 7_RStudio | n/a | n/a | 
Version History
Version 1 (earliest) Created 24th Jul 2025 at 10:16 by Myrthe van Baardwijk
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Created: 24th Jul 2025 at 10:16
Last updated: 22nd Aug 2025 at 11:24
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