Workflow Type: Common Workflow Language
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Amplicon analysis workflow using NG-Tax
Steps:
- Quality control on the reads
- Execute NGTax for ASV detection and classification
For more information about NG-Tax 2.0 have a look at https://doi.org/10.3389/fgene.2019.01366
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Inputs
| ID | Name | Description | Type |
|---|---|---|---|
| forward_reads | forward reads | forward sequence file locally |
|
| reverse_reads | reverse reads | reverse sequence file locally |
|
| forward_primer | Forward primer | Forward primer used |
|
| reverse_primer | Reverse primer | Reverse primer used |
|
| reference_db | Reference database | Reference database used in FASTA format |
|
| rev_read_len | Reverse read length | Read length of the reverse read |
|
| for_read_len | Reverse read length | Read length of the reverse read |
|
| sample | Sample name | Name of the sample being analysed |
|
Steps
| ID | Name | Description |
|---|---|---|
| fastqc | n/a | n/a |
| reads_to_folder | n/a | n/a |
| ngtax | n/a | n/a |
| fastqc_files_to_folder | n/a | n/a |
| ngtax_files_to_folder | n/a | n/a |
Outputs
| ID | Name | Description | Type |
|---|---|---|---|
| files_to_folder_fastqc | n/a | n/a |
|
| files_to_folder_ngtax | n/a | n/a |
|
Version History
Version 7 (latest) Created 28th Oct 2020 at 11:07 by Jasper Koehorst
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Creators and SubmitterCreators
Additional credit
Jesse van Dam, Peter Schaap,
Submitter
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Activity
Views: 12478 Downloads: 792
Created: 23rd Jul 2020 at 10:59
Last updated: 8th Jun 2021 at 08:11
AttributionsNone
Visit source
https://orcid.org/0000-0001-8172-8981