Workflow Type:  Common Workflow Language
        
        
        
  
            
              
                
                    
                    
              
            
        
          
            
              
    
      
        
        
    
    
      
        
        
    
    
      
        
        
    
            
          
        
        
      
  
    
      
        
      
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      Workflow Kallisto RNAseq
(pseudoalignment on transcripts)
- Workflow Illumina Quality: https://workflowhub.eu/workflows/336?version=1
- kallisto
All tool CWL files and other workflows can be found here:
Tools: https://git.wur.nl/unlock/cwl/-/tree/master/cwl
Workflows: https://git.wur.nl/unlock/cwl/-/tree/master/cwl/workflows
How to setup and use an UNLOCK workflow:
https://m-unlock.gitlab.io/docs/setup/setup.html
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Inputs
| ID | Name | Description | Type | 
|---|---|---|---|
| identifier | Identifier | Identifier for this dataset used in this workflow | 
 | 
| threads | Threads | Number of threads to use for computational processes | 
 | 
| memory | Maximum memory in MB | Maximum memory usage in megabytes | 
 | 
| filter_rrna | Filter rRNA | Optionally remove rRNA sequences from the reads. | 
 | 
| forward_reads | Forward reads | Forward sequence file locally | 
 | 
| reverse_reads | Reverse reads | Reverse sequence file locally | 
 | 
| kallisto_index | kallisto index file | kallisto index file location | 
 | 
| contamination_references | Reference filters files | Reference fasta file(s) for filtering (optional) | 
 | 
| destination | Output Destination | Optional Output destination used for cwl-prov reporting. | 
 | 
Steps
| ID | Name | Description | 
|---|---|---|
| workflow_quality | Quality and filtering workflow | Quality assessment of illumina reads with rRNA filtering option | 
| kallisto | kallisto | Calculates transcript abundances | 
| kallisto_files_to_folder | kallisto output | Preparation of kallisto output files to a specific output folder | 
Outputs
| ID | Name | Description | Type | 
|---|---|---|---|
| illumina_quality_stats | Filtered statistics | Statistics on quality and preprocessing of the reads | 
 | 
| kallisto_output | kallisto output | kallisto results folder. Contains transcript abundances, run info and summary. | 
 | 
Version History
Version 1 (earliest) Created 20th May 2022 at 11:10 by Bart Nijsse
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Created: 20th May 2022 at 11:10
Last updated: 2nd Nov 2022 at 15:26
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 https://orcid.org/0000-0001-8172-8981
 https://orcid.org/0000-0001-8172-8981

